Impact of Cassava Mill Effluent (CME) on soil physicochemical parameters microbial diversity and enzyme activities were analyzed in Akaeze, an agrarian community of Ebonyi State Nigeria. Results showed change in temperature (28.6-3260C), pH (7.2-10.3) and TOC (24.2-41.3 mg/g). Highest values were obtained nearest the waste pit while control soil had the least values. Total P, total N and C/N ratio were also lowest nearest the pit but cyanogenic potential was highest nearest pit. The soil heavy and trace metals (Hg, Cr, Pb andCd) were not affected except Ca which was highest near the pit and decreased away from it. Total heterotrophic bacteria had a count range of 3.7 x104-6.6 x106 cfu/g, lipolytic bacteria, had 0.9 x101-24x103 cfu/g while nitrifying bacteria had 0.4-2.9 x3. 7x103 cfu/g. Phosphate-solubilizing bacteria had a range of 2.2 x102-2.4 x103 cfu/g. In all cases, highest values were obtained 100m