The effect of Palm Oil Mill Effluent (POME) on the physicochemical and biological properties of Petroleum impacted Orashi-River Wetland soil was investigated in Egbema. Varying amounts (10L, 15L, 20L, and 25L) of petroleum were applied on plots of 3m 2 uniformly before amending with 5L of POME. The POME had 0.83%(NO 3), PO 4 0.513), Total Solids (37mg) and TOC (9.4). The metallic elements were K (0.30 mgKg-1), Ca (0.23 gKg-1) and Mg (0.50 mgKg-1). The soil metallic components (Mg, Ca and K) and temperature were statistically unchanged after 90 days bioremediation time (P= 0.05). The organic matter, total NO3, and total PO4 were significantly increased after bioremediation above the control soil values. The populations of bacterial groups (Total Heterotrophic Bacteria (THB); Nitrifying bacterial (NB), Phosphate Solublizing Bacteria (PSB), Petroleum Utilizing (PUB) and Lipolytic Bacteria (LB) decreased significantly after oil impaction and amendment but rose above the control after the remediation period. Though bacterial loads of 20L and 25L impacted soil improved, they were still below those of the control soil. The enzymatic activities followed the bacterial population trend. Dehydrogenase activity decreased with the population of the producing organisms (THB) just like lipase (LB). Phenol oxidase and the phosphatase correlated positively with changes in pH values. Urease activity was correlated positively with the nitrifying bacterial count. 10L oil impacted soil showed the best remediation status followed by the 15L oil impacted soil while the above 20L oil impacted areas still had values significantly below the control. POME